Blackleg PCR Kit

Background Information

Blackleg is caused by infection with Clostridium bacteria. The most common causitive agent is C. chauvoei, but the disease can also be caused by C. septicum, C. sordelli, and C. novyi. C. chauvoei is Gram-positive, rod-shaped, anaerobic, and motile, and can produce environmentally persistent spores when conditions are not ideal for growth. These spores can remain in the soil for years in an inactive state, and return to their infectious form when consumed by grazing livestock. Contaminated pasture is a predominant source of these organisms, which are also found naturally in the intestines of animals. Cases can occur over many years in areas where soil or manure is contaminated with the bacteria, and it is exceedingly difficult to remove the spores from the environment. C. chauvoei can produce a large amount of gas as a metabolic byproduct when growing and reproducing, hence the alternate name gas gangrene, present in humans. This gas builds up in infected tissue, usually large muscles, and causes the tissue to make a crackling or popping sound when pressed. Large gas-filled blisters can also form, which can be extremely painful as they build up in the tissues.

LiliF™ Blackleg PCR Kit can directly detect the protozoa in Blackleg spp. Based on the genetic database and diagnose it very quickly and accurately. Using the polymerase chain reaction (PCR) within 2 ~ 3 hours, It is a very rapid and reliable way to amplify genes only. Each tube in the kit contains polymerase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR, except template for gene specific amplification for Blackleg disease. It is very easy to add template and template.