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FHV PCR Kit

High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions 94 KDa thermostable DNA polymerase

  • High purity Taq DNA Polymerase
    – Removal of E. coli -derived proteins and DNA that may act as PCR sources
  • Applicable to DNA from cloned DNA to human genomic DNA
  • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • Pack Promo 5×500 units.Código: 25022-5

 

Availability:

18 disponibles

199,00

18 disponibles

Background Information

Feline rhinotracheitis virus (feline herpesvirus type 1 or FHV-1) causes acute respiratory illness known as rhinotracheitis (or feline herpesvirus infection). The virus affects domestic and wild cats worldwide. Rhinotracheitis is characterized by respiratory symptoms such as sneezing, nasal discharge, rhinitis (inflammation of the nose), and conjunctivitis (inflammation of the membrane lining the eyelid). It also affects the reproductive tract and can cause complications during pregnancy. Rhinotracheitis is part of the feline upper respiratory infection complex, which is a group of viral and bacterial infections (e.g., calicivirus, chlamydiosis) that cause sneezing and discharge from the eyes and nose. Cats often have two or more of these upper respiratory infections at the same time, and FHV-1 is one of the most common. FHV-1 can be transmitted by direct contact with an infected cat’s mouth, nose, or eye discharge. Several days of close contact are necessary for infection to occur.

LiliF™ FHV PCR Kit is able to detect directly and specifically feline herpesvirus by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of feline herpesvirus, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.

• This product is optimized for a general purpose PCR machine with a 0.2 ml PCR tube thermal block. Based on the genetic database of the causative feline herpesvirus, the reaction product is formed by specific primers of Feline immunodeficiency virus. After completion of the reaction, confirm whether a band of target size is detected using an electrophoresis and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 FHV Detection Premix 48 tubes
2 FHV Positive Control 25µl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube

Descargar protocolo

 IDR-RP0040_i-Taq.pdf

SKU: IP11176 Categorías: , Etiqueta:
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