Myco-P PCR Kit

High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions 94 KDa thermostable DNA polymerase

  • High purity Taq DNA Polymerase
    – Removal of E. coli -derived proteins and DNA that may act as PCR sources
  • Applicable to DNA from cloned DNA to human genomic DNA
  • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • Pack Promo 5×500 units.Código: 25022-5

 

Availability:

18 disponibles

199,00

18 disponibles

Background Information

Mycoplasma hyopneumonia the causative agent of enzootic pneumonia and plays an important role in the porcine respiratory disease complex (PRDC). The chronic form of the disease affects up to 80% of pigs around the world and is characterized by high morbidity and low mortality rates. As a single infection, it causes enzootic pneumonia, a prevalent and persistent respiratory disease of swine. In combination with other respiratory pathogens, i.e., porcine reproductive and respiratory syndrome virus (PRRSV) or swine influenza virus, however, pneumonia due to the second pathogen is significantly enhanced. Vaccination against M. hyopneumoniaedoes not prevent its colonization of the swine respiratory tract or protect sufficiently against disease, nor does it obviate the potentiating role of M. hyopnuemoniaein dual infection with other infectious pathogens. It is impossible to diagnose accurately by clinical symptoms alone, and various diseases and differential diagnosis are needed. Since the large number of bacteria are contained in the sputum at the early stage of the onset, it is possible to detect the gene directly f rom the sputum or to isolate causative bacteria of pneumonia f rom spleen latex or cell f rom lung lesion Because the incubation time is usually 1 to 2 weeks, it is not suitable for early diagnosis and requires a very long time, so molecular diagnosis is required for rapid diagnosis.

LiliF™ Myco-P PCR Kit was designed to detect mycoplasma hyopneumoniae directly based on genetic database and to diagnose it very quickly and accurately. Only 3 hours after amplification, specific gene was amplified using polymerase chain reaction (PCR) It is very fast and reliable. Each tube in the kit contains polymerase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR except template for Mycoplasma hyopneumoniae gene specific amplification, It is very easy to add template and template.

Principle

• This product is an in vitro diagnostic reagent for animal which is qualitatively detected by polymerase chain reaction (PCR) to detect Mycoplasma hyopneumoniae pathogen f rom animal sputum, spleen latex or cell f rom lung lesion cells.

• This product is optimized for universal PCR machine with 0.2 ml PCR tube thermal block.

• Based on Mycoplasma hyopneumoniae genetic database, reaction product is formed by specific primer of Mycoplasma hyopneumoniae.

• Use an electrophoresis device and agarose gel to check if the band of the target size is detected.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 Myco-P PCR Premix 48 tube
2 Positive Control 25 μl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube

Descargar protocolo

 IDR-RP0040_i-Taq.pdf

SKU: IP21424 Categoría: Etiqueta:
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