BVDV Real-time RT-PCR Kit

Background Information

Bovine viral diarrhea or bovine viral diarrhoea, and previously referred to as bovine virus diarrhoea (BVD), is a significant economic disease of cattle that is endemic in the majority of countries throughout the world. The causative agent, bovine viral diarrhea virus (BVDV), is a member of the Pestivirus genus of the family Flaviviridae. BVD infection results in a wide variety of clinical signs, due to its immunosuppressive effects, as well as having a direct effect on respiratory disease and fertility. In addition, BVD infection of a susceptible dam during a certain period of gestation can result in the production of a persistently infected fetus. Pestiviruses are small, spherical, single-stranded, enveloped RNA viruses of 40 to 60 nm in diameter. The genome consists of a single, linear, positive-sense, single-stranded RNA molecule of approximately 12.3 kb.

LiliF™ BVDV Real-time RT-PCR Kit is able to detect directly and specifically BVDV 5’-UTR by real-time PCR technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer and probe set for a highly conserved region based on current sequence alignments of all type of BVDV 5’-UTR allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the real-time RT-PCR (quantitative) method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.