- Antibioticos&Biochemicals, DNA Kits, DNA/RNA, Genomicos
Proteinasa K Grado Biología Molecular
Proteinasa K produced by the fungus Tritirachium album Limber; is a serine protease with broad cleavage activity. Proteinasa K cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples. It has been purified to remove RNase and DNase activities. The stability of Proteinasa K in urea and SDS and its ability to digest native proteins make it useful for a variety of applications including preparation of chromosomal DNA for pulsed-field gel electrophoresis, protein fingerprinting and removal of nucleases from preparations of DNA and RNA. A typical working concentration for Proteinasa K is 50–100μg/ml.
Form: Lyophilized powder.
Recommended Reaction Buffer: 50mM Tris-HCl (pH 8.0), 10mM CaCl2.
Código: 405002 para 200mg
Código: 405003 para 1gSKU: 405002