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FIV PCR Kit

Availability:

18 disponibles


High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions 94 KDa thermostable DNA polymerase

  • High purity Taq DNA Polymerase
    – Removal of E. coli -derived proteins and DNA that may act as PCR sources
  • Applicable to DNA from cloned DNA to human genomic DNA
  • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • Pack Promo 5×500 units.Código: 25022-5

 

199,00

18 disponibles

Background Information

Feline immunodeficiency virus (FIV) causes an infectious disease in domestic cats and cheetahs similar to human immunodeficiency virus (HIV infection) in humans. It attacks and weakens the body’s immune system, making the animal susceptible to infections and diseases that don’t affect healthy cats. There is neither a cure nor a vaccine for FIV. Though eventually fatal, an FIV-positive cat can live for many years without any signs of illness. FIV is transmitted primarily through deep, penetrating bite wounds. A mother cat may transmit the virus to her newborn kittens during gestation, passage through the birth canal, or nursing. FIV can also be transmitted through the transfusion of contaminated blood. FIV affects only felines. Some of the pathogens (i.e., bacteria, parasites) that cause opportunistic infections in FIV-positive cats may be transmitted from animals to humans and could cause illness in people with compromised immune systems.

LiliF™ FIV PCR Kit is able to detect directly and specifically Feline immunodeficiency virus by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Feline immunodeficiency virus, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.

• This product is optimized for a general purpose PCR machine with a 0.2 ml PCR tube thermal block. Based on the genetic database of the causative Feline immunodeficiency virus, the reaction product is formed by specific primers of Feline immunodeficiency virus. After completion of the reaction, confirm whether a band of target size is detected using an electrophoresis and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No.contents48 tests/kit
1FIV Detection Premix48 tubes
2FIV Positive Control25µl x 3 tubes
3DNase/RNase Free Water1 ml x 1 tube

Descargar protocolo

 IDR-RP0040_i-Taq.pdf

SKU: IP11177 Categorías: , Etiqueta: