Antibody based-Hot Start method, i-StarTaq ™ GH DNA Polymerase with high specificity and high amplification efficiency
• high specificity and accuracy of antibody based Hot-start method
• Optimal Buffering System for High Amplification Efficiency
• High purity Hot-Start Taq DNA Polymerase
• Highly reproducible results
• Suitable products for various applications requiring high amplification efficiency and specificity
In general PCR, the activity of Taq DNA polymerase can be increased to 20 ~ 30 ℃ at room temperature. Therefore, before the first denaturation process is completed, It may happen. If annealing occurs at such a low temperature, there is a high probability that the primer will mismatch, resulting in a band that is less sensitive and less specific than the desired PCR product. Therefore, Hot-Start Taq DNA Polymerase is used to prevent this phenomenon. There are many ways to implement hot-start. I-StarTaq ™ GH DNA Polymerase is produced by using antibody in these methods. The i-StarTaq ™ GH DNA Polymerase uses an antibody-based Hot Start method to block the enzyme activity at low temperature. As the temperature of the reactant rises, the enzyme becomes active as the temperature of the reactant increases, causing non-specific priming or oligomerization You can stop PCR and perform very good results. In addition, the optimal buffering system has excellent sensitivity and amplification rate. Therefore, it can be widely used for post-PCR experiments such as cloning and for food environmental hygiene inspection, genome analysis and gene diagnosis requiring high sensitivity. PCR is performed with a trace amount of gDNA extracted from tissues It can also be used in the field of forensic medicine using genetic fingerprinting, such as hospital laboratories, evolutionary biology that analyzes the genetic makeup, paternity identification, and criminal identification. In addition, i-StarTaq ™ GH DNA Polymerase are used for mutation analysis, diagnosis of genetic diseases, identification of viruses and various pathogens.
Observation of amplification efficiency of various hot-start PCR conditions
Based on various PCR amplification conditions, we compared the amplification efficiency with that of our existing product, i-StarTaq™ DNA Polymerase. As a result, we were able to confirm that the sensitivity and amplification rate are better than those of existing products.
Fig 1. PCR amplification of various PCR Results with i-StarTaq™ GH DNA Polymerase and i-StarTaq™ DNA Polymerase
[ Panel A ] GAPDH (570 bp); [ Panel B ] 1.3 Kb amplification; [ Panel C ] 1.8 Kb amplification
[ Panel A/B/C ]
Lane M, SiZer™-100 plus DNA Marker Solution(Cat. No. 24072); lane N, Negative Control
Lane 1, 100 ng K-562 gDNA; lane 2, 10 ng K-562 gDNA; lane 3, 1 ng K-562 gDNA; lane 4, 100 pg K-562 gDNA; lane 5, 10 pg K-562 gDNA; lane 6, 1 pg K-562 gDNA; lane 7, 100 fg K-562 gDNA
Examples of molecular diagnostics
i-StarTaqTM GH DNA Polymerase was applied to the molecular diagnosis to compare sensitivity and amplification with the existing i-StarTaq™ DNA Polymerase.
Fig 2. PCR amplification of Molecular diagnosis PCR Results with i-StarTaq™ GH DNA Polymerase and i-StarTaq™ DNA Polymerase
[ Panel A ] Salmonella spp. Detection; [ Panel B ] FHV Detection
[ Panel A ], Lane M, SiZer™-100 plus DNA Marker Solution(Cat. No. 24072); lane 1 ~ 7, 1/10 serial diluted Salmonella spp. DNA; lane N, Negative control
[ Panel B ], Lane M, SiZer™-100 plus DNA Marker Solution(Cat. No. 24072); lane 1 ~ 7, 1/10 serial diluted FHV DNA; lane N, Negative control