PRRSV RT-PCR Kit

Background Information

The virus in the Arteriviridae group is the cause of the disease at an unclear stage. This virus has an outer membrane, a small spherical virus with a diameter of 45-80 nm, and small protrusions on the surface. Pigs can be infected with PRRS (Porcine reproductive & respiratory syndrome) viruses through nasal passages, muscles, oral, uterine and abdomen, and pigs are generally more susceptible to parenteral infections than oral ones. Pig reproductive and respiratory syndrome has two distinct clinical features, reproductive disorder and respiratory disease. In the breeding stage, the infant of piglets, the embryo of fetus, premature birth and birth of fragile pigs increase. Anorexia and no manifestation are increased (30-50%) in nursing pigs. As a suckling young pig grows, it exhibits a distinct respiratory pattern and pathologic examination results in severe necrosis and pneumonia in the lung tissue. Diagnosis is usually performed using serological tests, virus isolation, virus detection, etc., and differential diagnosis with Japanese encephalitis, parvovirus infection, myocarditis virus infection, Ozeki disease is necessary. Pig genital respiratory syndrome is a viral disease, but there is no cure, but antibiotics will help to relieve symptoms and treat secondary bacterial infections.

The LiliF ™ PRRS RT-PCR Kit was designed to detect very rapidly and accurately the nonstructural proteins of genital respiratory syndrome virus based on a genetic database. RT-PCR, reverse transcription -Polymerase Chain Reaction) to amplify only specific gene, which is a very fast and reliable method. Each tube in the kit contains polymerase, reverse transcriptase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR, except templates for pig genital respiratory syndrome virus gene specific amplification. It is very easy to add only distilled water and template for RT-PCR.