Using RunVIEW is simple; first, cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied, before transferring the blue-light transparent gel tray to the MSCHOICE tank located on the base unit. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the upper row of wells (loading tier). Replace the lid to connect the MSCHOICE tank to the integrated power supply before applying the voltage and switching on the blue LED gel illuminator. Watch through the bluVIEW lid viewing pane as the samples migrate in real-time to the second row of wells (extraction tier). Once the DNA bands of interest enter the tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.
Track DNA without harmful UV
Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning efficiency. RunVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.
Save on time-consuming gel elution techniques
Running DNA into a well containing buffer effectively purifies the DNA of agarose, eliminating the need for time-consuming gel excision and purification techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.
No expensive commercial gels
RunVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the 15×7, 15×10 or 15x15cm MSCHOICE gel trays, and therefore does not require expensive precast gels and accessories.
A self-contained system
The base unit, which houses the in-built power supply and blue LED gel illuminator, is compact, dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction to be performed at the bench, without the inconvenience of having to transport gels to a darkroom elsewhere within the laboratory.