SIV-NP RT-PCR Kit

Background Information

Swine influenza (Swine influenza) is an acute respiratory infectious disease caused by swine influenza virus, which is one of the influenza A viruses. Swine influenza causes the swine to develop suddenly and symptoms such as cough, dyspnea, fever and loss of energy. It is characterized by recovery in 5-7 days and mortality rate is less than 1%. The disease is spread all over the world and the seasonal rate of infection is not very clear, but the incidence is somewhat high between late autumn and early spring. The pathogen of swine influenza virus is the influenza A virus (H1N1, H1N2, H3N2). Influenza viruses are divided into three types of A, B, and C based on antigenic differences between nucleoprotein (NP) and matrix (M) proteins. Among them, influenza A viruses are classified as hemagglutinin HA) and neuraminidase (NA) glycoproteins. Influenza A viruses can be seen in people, pigs, horses, and birds. Type B and type C can be seen only in humans. The disease was first diagnosed in a pig farm in Illinois, USA in 1918, when the first outbreak occurred in a pig of millions of dollars and killed several thousand. It is also reported that more than 20 million people have died of influenza, which is called the Spanish flu on a global scale. Pandemic influenza occurs independently of the sex and age of pigs, but seasonal factors are known to have a major impact. Thus, most occurrences occur during cooler periods, f rom late autumn to early spring. Also, on farms where this disease has occurred f rom late autumn to early spring, this disease usually does not occur f rom autumn to the following epidemic.

The LiliF ™ SIV-NP RT-PCR Kit was designed to detect very rapidly and accurately the nonstructural proteins of swine influenza virus based on a genetic database. RT-PCR, reverse transcription -Polymerase Chain Reaction) to amplify only specific genes, which is a very fast and reliable method. Each tube in the kit contains polymerase, reverse transcriptase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR, except templates for swine influenza virus gene specific amplification. It is very easy to add only distilled water and template for RT-PCR.