2x PCR Master mix type with high sensitivity, accuracy and low error rate, capable of amplification from small size DNA to DNA of more than 20 Kb with a proofreading function
• Ready to Use
– All reagents required for the reaction are in solution in the tube
– Immediate PCR by adding template and primer
• PCR reaction volume control
• High accuracy and low error rate
– Proof-reading activity enables amplification of long DNA template
– Ensures high amplification efficiency for amplification of long and short template DNA
• Cloned DNA, human genomic DNA, as well as GC-rich or looped sequences that are difficult to amplify AND applicable to various DNA
2x PCR Master Mix Solution (i-MAXTM II) is a product designed to achieve the best results with simple 2x master mix type in one tube, including all components necessary for PCR reaction including i-MAXTM II DNA Polymerase is. This product is able to perform PCR by adding template DNA, primer set and D.W only, and gel loading buffer is included, so gel loading can be done without any other treatment. The i-MAXTM II DNA Polymerase used in this product has the advantages of Taq DNA Polymerase and Pfu DNA Polymerase to overcome the disadvantages of each enzyme and to obtain new functions. It has a proofreading function, Kb) to 20 Kb or more.
The advantages and disadvantages of each enzyme are as follows. In the case of Taq DNA Polymerase, if the template is over 5 Kb, the efficiency of amplification is significantly lowered. Depending on the type of template, DNA template of 10 Kb or more is difficult to amplify. This is because Taq DNA Polymerase has no proof-reading activity, and when mismatched nucleotides are inserted during DNA synthesis, the template fails to polymerize. These defects in Taq DNA Polymerase can be overcome by using a thermostable DNA polymerase with corrective function. This corrective function is to remove the mismatched (non-complementary) nucleotide when the DNA Polymerase undergoes a polymerization reaction, and correct it with a complementary nucleotide. This activity allows PCR products of more accurate base sequences to be obtained than Taq DNA Polymerase, but allows amplification of longer DNA templates compared to Taq DNA Polymerase.
Pfu DNA Polymerase is a typical enzyme of thermostable DNA Polymerase with this corrective function. It is suitable for long size PCR (up to ~ 30 Kb), which is difficult to amplify with general Taq. It has higher fidelity and proof reading function than general Taq DNA Polymerase . However, Pfu DNA Polymerase has the disadvantage that it takes more time to polymerize the DNA due to its poor polymerization efficiency. As mentioned above, the 2x PCR Master Mix Solution (i-MAXTM II) uses i-MAX ™ II DNA Polymerase, which takes advantages of Taq DNA Polymerase and Pfu DNA Polymerase, It is a product that can increase the amplification rate in size, show excellent results in sensitivity part, reproducibility is high, and can be tested quickly and easily on various samples.
Sensitivity Comparison data
In order to compare the sensitivity of 2x PCR Master Mix Solution (i-MAX TM II) with other products of the same function, 1 Kb DNA fragment PCR was performed by diluting human genomic DNA template with concentration. It shows that our kit obtained better result.
A, Competitor; B, 2x PCR Master mix Solution(i-MAXTM II )
Lane M, 1 Kb DNA Marker; lane NC, Negative control; lane 1, 50 ng gDNA, Lane 2, 25 ng gDNA; lane 3, 12.5 ng gDNA; lane 4, 6.25 ng gDNA; lane 5, 3.125 ng gDNA; lane 6, 1.56 ng gDNA; lane 7, 0.753 ng gDNA
In order to compare the sensitivity of 2x PCR Master Mix Solution (i-MAXTM II) with other products of the same function, long PCR of 20 Kb fragment was performed by diluting λDNA template by concentration. It shows that our kit obtained better result.
A, 2x PCR Master mix Solution(i-MAXTM II); B, Competitor
Lane M, 1 Kb DNA Marker; lane N, Negative control; lane 1, 50 ng DNA; lane 2, 25 ng DNA, lane 3, 12.5 ng DNA, lane 4, 6.25 ng DNA, lane 5, 3.125 ng DNA; lane 6, 1.56 ng DNA; lane 7, 0.753 ng DNA