dNTP Mix 100mM
dNTP Mix 1ml/100mM Ultrapure
Applications for dNTP mix and dNTP – sets:
– all molecular biology applications
– PCR/qPCR
– reverse transcription
– DNA labeling
– DNA sequencing
ALL dNTP’s are free of human or bacterial DNA
SKU: 25002
18 disponibles
45,00€ 59,00€
18 disponibles
dNTP Mix Ultrapure as set of 100 mM
Applications for dNTPs mix and dNTPs-sets:
– all molecular biology applications
– PCR/qPCR
– reverse transcription
– DNA labeling
– DNA sequencing
ALL dNTP are free of human or bacterial DNA
Description dNTP-mix and dNTP-sets:
dNTP-sets from Intronbio have PCR Grade and contains four separate tubes of dATP, dCTP, dGTP and dTTP supplied as aqueous solutions at pH 8.5. The dNTP-Mix contains an optimized mixture of dNTP`s with 10 mM of each nucleotide.
Quality control dNTPs mix and dNTPs-set:
– purity: purity test with RP-HPLC: greater 99 % (stable from lot-to-lot)
– Applification tests: Lambda DNA: 18 kb fragment, less than 20 pg template
RT-PCR: Human DNA, 600 bp fragment, less than 20 pg template
– Free of bacterial – and human DNA
– Free of: DNase, RNase, Protease and no nicking activity
– for all tests please see the dNTP-Test-sheet at the end or the side
dNTPs set: Quality control and Purity tests
Purity of dNTPs is a big factor in PCR performance. A specifcation of ≥ 99.0 % dNTP is the market standard. But the purity is not the the most importand point. The constitution of the remaining ≤ 1.0 % is crucial.
Common contaminants in dNTPs solutions are:
– Deoxyribonucleotide diphosphates – dNDPs
– Ribonucleotides – NTPs
– Other dNTPs – e.g. deaminated or methylated dNTPs
– Other nucleosidic compounds
– Phosphates
Even trace quantities of other impurities can signifcantly affect performance, hence our aim is to minimize all traces.
dNTP Set: Composition
| dATP 2′-Deoxyadenosine 5′-triphosphate, sodium salt CAS Number: 1927-31-7 Formula: C10H13N5O12P3 (Anion) Molecular weight: 488.16 g·mol-1 Concentration: 100 mM | dCTP 2′-Deoxycytidine 5′-triphosphate, sodium salt CAS Number: 102783-51-7 Formula: C9H13N3O13P3 (Anion) Molecular weight: 464.13 g·mol-1 Concentration: 100 mM |
| dGTP 2′-Deoxyguanosine 5′-triphosphate, sodium salt CAS Number: 93919-41-6 Formula: C10H13N5O13P3 (Anion) Molecular weight: 504.16 g·mol-1 Concentration: 100 mM | dTTP 2′-Deoxythymidine 5′-triphosphate, sodium salt CAS Number: 18423-43-3 Molecular formula: C10H14N2O14P3 (Anion) Molecular weight: 479.14 g·mol-1 Concentration: 100 mM |
dNTP-lot test series
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Taq Polimerasa con dNTP´s
Taq Polimerasa is High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions
- 94 KDa thermostable DNA polymerase
- High purity Taq DNA Polymerase
– Removal of E. coli -derived proteins and DNA that may act as PCR sources - Applicable to DNA from cloned DNA to human genomic DNA
- Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
- Presentación: Vial 500 Units / 5U/ul
- Código: 25022
SKU: 25022
