Double-RNA Viral dsRNA Extraction Mini Kit (for Plant Tissue)
Spin type product for extracting viroid, pathogenic plant virus in a short period
• Possible to extract viroid, pathogenic plant virus selectively within 30 minutes
• Possible to purify dsRNA selectively
• CsCl gradient, LiCl alcohol PPT are not necessary
• Useful for molecular diagnostic due to minimizing concerns for contamination by using CAPS (Column) method
Double-RNA Viral dsRNA Extraction Mini Kit(For Plant Tissue)is designed to extract only double-stranded virus RNA that are 10% among plant disease infectious virus. Most plant pathogenic viruses contain single-strand RNA but it will change form to double-strand RNA during replication. These dsRNA usually binds to cellulose under low alcohol concentration. Gene extraction under low concentration of alcohol can lead to a large amount of pathogenic ssRNA based on high concentration of salt with binding Silica- based gene and a co-precipitation may occur depending on the type and amount of the sample. Therefore, this product is possible for selective dsRNA extraction in a short time because it can maximize the reproducibility of experiment by preventing column clogging. In addition, this product can be safely used because it does not require any organic solvents or ethanol precipitation. It can easily extract genes within 20 minutes and CAPS method is applied to the column to minimize the concerns for contamination.
Sensitivity data for pathogenic plant virus detection
RT-PCR was performed using extracted double-RNA by Double-RNA Viral dsRNA Extraction Mini Kit(As you see above, efficiency of extraction with Double-RNA Viral dsRNA Extraction Mini Kit For Plant Tissue was higher compare to others.
iNtRON, purified RNA with Double-RNA Viral dsRNA Extraction Mini Kit; Manual; purified RNA with manual method A, potato X virus(PVX); B, potato leaf roll virus(PLRV); C, potato Y virus(PVY); D, potato S virus(PVS) Lane M, 100bp ladder; lane 1, 20 ㎕ of RNA used as template of RT-PCR; lane 2, 10 ㎕ of RNA used as template of RT-PCR); lane 3, 5 ㎕ of RNA used as template of RT-PCR; lane 4, 2.5 ㎕ of RNA used as template of
Comparison data between Double RNA and total RNA (Potato X virus )
After serial dilution was performed with extracted RNA using the Double-RNA Viral dsRNA Extraction Mini Kit (For Plant Tissue) and manual method, PVX was confirmed with Maxime RT-PCR PreMix Kit (iNtRON, Cat. No. 25131) . As shown from the results, it can be seen that the extracted RNA is higher yield than the manual extracted RNA.
Lane M, 100 bp Ladder; lane N, No template control; lane 1, 0.5 ㎕ of extracted RNA used as RT-PCR template lane 2~7, 0.5 ㎕ of 1/10 serially diluted RNA used as RT-PCR template from 10-1 to 10- 6