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FCV RT-PCR Kit

Availability:

18 disponibles


High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions 94 KDa thermostable DNA polymerase

  • High purity Taq DNA Polymerase
    – Removal of E. coli -derived proteins and DNA that may act as PCR sources
  • Applicable to DNA from cloned DNA to human genomic DNA
  • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • Pack Promo 5×500 units.Código: 25022-5

 

199,00

18 disponibles

Background Information

Feline calicivirus (FCV), member of the family Caliciviridae, genus Vesivirus is a major cause of acute and chronic upper respiratory diseases in cats. Commonly seen clinical signs include rhinitis, conjunctivitis, stomatitis, gingivitis and glossitis. Less often pneumonia, fever, abortion, cystitis and limping are seen. After resolution of the infection, apparently healthy cats may shed virus for several months or even years. Prevalence rates of feline calicivirus in cats without clinical symptoms vary between 2.5 and 25%. These asymptomatic carrier cats are believed to contribute to the epidemiology of feline calicivirus infection and disease. Recently, virulent feline calicivirus strains associated with a hemorrhagic-like fever and mortality up to 60% in adult cats have been described. Feline calicivirus vaccines have been used widely in the field for the last 20 years. Despite these efforts, feline calicivirusrelated illnesses still present a major problem in the feline population especially for cats living in groups.

LiliF™ FCV RT-PCR Kit is able to detect directly and specifically calicivirus by CLP™ technology and Maxime™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of calicivirus, allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional RT-PCR method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative RT-PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes RT-PCR specificity and sensitivity through the control of non-specific priming.

• The assay is a RT-PCR that discriminates calicivirus in one reaction. The assay is composed of two principal steps: (1) nucleic acid extraction from specimens, and (2) amplification of the target extracted nucleic acid fragment using specific primers pair.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No.contents48 test/kits
1FCV Detection Premix48 tubes
2FCV Positive Control25 μl x 3 tubes
3DNase/RNase Free Water1 ml x 1 tube

Descargar protocolo

 IDR-RP0040_i-Taq.pdf

SKU: IP12177 Categorías: , Etiqueta: