JEV Genotype 1&3 RT-PCR Kit

Background Information

Japanese encephalitis (JE) is an infection of the brain caused by the Japanese encephalitis virus (JEV).While most infections result in little or no symptoms, occasional inflammation of the brain occurs. In these cases symptoms may include headache, vomiting, fever, confusion, and seizures. This occurs about 5 to 15 days after infection.EV is generally spread by mosquitoes, specifically those of the Culex type.Pigs and wild birds serve as a reservoir for the virus. The disease mostly occurs outside of cities. Diagnosis is based on blood or cerebrospinal fluid testing. Prevention is generally with the Japanese encephalitis vaccine, which is both safe and effective. Other measures include avoiding mosquito bites. Once infected there is no specific treatment, with care being supportive. This is generally carried out in hospital. Permanent problems occur in up to half of people who recover from JE.The disease occurs in Southeast Asia and the Western Pacific About 3 billion people live in areas where the disease occurs About 68,000 symptomatic cases occur a year with about 17,000 deaths. Often cases occur in outbreaks. The disease was first described in 1871.

The LiliF™ JEV Genotype 1 & 3 RT-PCR Kit was designed to detect the genes associated with the encephalitis virus proteins directly and to detect them very quickly and accurately. Based on the genetic database, RT-PCR Kit (RT-PCR, Reverse Transcription -Polymerase Chain Reaction) to amplify only specific genes, which is a very fast and reliable method. In order to simultaneously detect the specific amplification of the Japanese encephalitis virus genotype 1 and 3 genes, each tube in the kit was treated with polymerase, reverse transcriptase, dNTPs, 10x reaction buffer, RNase inhibitor for hot start PCR, template specific primer And tracking dyes. It is very easy to add only distilled water and template for RT-PCR.