PCV2 PCR Kit

Availability:

18 disponibles


High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions 94 KDa thermostable DNA polymerase

  • High purity Taq DNA Polymerase
    – Removal of E. coli -derived proteins and DNA that may act as PCR sources
  • Applicable to DNA from cloned DNA to human genomic DNA
  • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • Pack Promo 5×500 units.Código: 25022-5

 

199,00

18 disponibles

Background Information

Pig circovirus is the causative agent of systemic wasting syndrome after infectious congenital progression (PMWS). In the case of infectious congenital developments, the clinical symptoms of infected piglets can be quite variable even in piglets. F rom mild to severe progression, the cause of death is starved to death because piglets within one week of age can not feed. Systemic wasting syndrome occurs in 6 to 16 weeks of age, when the maternal antibody is lost in piglets. Especially in pigs aged 8 to 12 weeks, infected pigs become chronic cysts, pale skin and lymph nodes It is characterized by bigger. In some infected pigs, respiratory symptoms such as dyspnea and chronic pneumonia, and diarrhea and gastric symptoms such as gastric ulcers may be present. Another important feature is the slow progression of the disease, so it can be confused with other diseases by slowly developing the disease. Infection rates vary f rom one to two, but mortality rates can be high (5-50%). When the lesion is confirmed, the lymph nodes of the spleen and the whole body are 3-4 times larger, and the spots can be observed in the lung. The kidneys may have white spots, pale and edema, which can be up to five times larger than normal. Histopathologic findings include lymphocyte deficiency and interstitial pneumonia. There are no gross lesions in pigs showing congenital progress. In the case of postprandial systemic wasting syndrome, clinical symptoms such as weight loss, mastication, typical atrophy, dyspnea and jaundice as well as diarrhea, cough and central nervous system anxiety are seen. For precise diagnosis, a molecular biology test using a PCR method is necessary to isolate virus using tissue cultured cells or to detect two types of PCV.

The LiliF™ PCV-2 PCR Kit was designed to detect rapidly and accurately the nonstructural proteins of porcine circovirus virus based on a genetic database. PCR was performed within 2 hours using Polymerase Chain Reaction It is characterized by its ability to amplify only specific genes, making it a very fast and reliable method. Each tube in the kit contains polymerase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR except template for specific amplification of porcine circovirus gene. It is very easy to add only distilled water and template.

Principle

• This product is an in vitro diagnostic reagent for animals that detects qualitatively by polymerase chain reaction (PCR) in order to detect porcine circovirus virus pathogen f rom animal body tissues.

• In other words, it is a product that can qualitatively diagnose quickly by adding a positive control substance, DNase / RNase free water, supplied f rom the viral DNA extracted f rom veterinary body tissues, etc., using viral gene extraction kit.

• This product is optimized for a general purpose PCR machine with 0.2 ml PCR tube thermal block.

• Based on the genetic database of pig swine virus, reaction products are formed by specific primers of porcine circovirus After completion of the reaction, use an electrophoresis apparatus and agarose gel to check whether a band of target size is detected.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only.

• It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No.구성물48 tests/kit
1PCV-2 Detection Premix48 tubes
2PCV-2 Positive Control25 µl x 3 tubes
3DNase/RNase Free Water1 ml x 1 tube

Descargar protocolo

 IDR-RP0040_i-Taq.pdf

SKU: IP11527 Categoría: Etiqueta: