RedSafe™ Nucleic Acid Staining Solution

Description

The RedSafe™ Nucleic Acid Staining Solution exhibits two fluorescence excitation maxima at 309 nm and 419 nm when bound to nucleic acids and is visible at a wavelength of 514 nm. RedSafe™ Nucleic Acid Staining Solution is as sensitive as EtBr and its usage is similar to EtBr. Compared with EtBr, a potent carcinogen, RedSafe™ Nucleic Acid Staining Solution showed almost no mutation in the Ames test and negative results in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. Therefore, we strongly recommend that you select RedSafe™ Nucleic Acid Stainig Solution (20,000x) instead of EtBr to identify nucleic acids on agarose gel.

Characteristics

• RedSafe™ Nucleic Acid Staining Solution is easy and convenient to use, offering higher sensitivity than EtBr.

• It has significantly lower genotoxicity compared to EtBr, ensuring user and laboratory safety.​

• When bound to nucleic acids, it becomes visible at 520–540 nm (optimal with a 530 or 535 nm filter).

Application

• Visualization of DNA and RNA bands during agarose gel electrophoresis.

• Isolation of DNA fragments for subcloning while minimizing mutation risks associated with EtBr.

• Both Precasting and Post-Staining are all possible.

Storage

• RT, Room Temperature : Stable for more than 12 months

• For more stable use, should be store at 4℃ (Stable for more than 24months).

Technical Data

1. Spectrum

 

[Excitation (EX) and Emission Wavelengths]

• EM scan at 306 nm EX showed the highest center peak at 530-535 nm.

• EX scan at 535 nm EM revealed multiple peaks, with the center peaks at 270 nm, 300 nm, 480 nm, and 495 nm.

[Excitation Capabilities]

• RedSafe can be excited not only by the UV light typically generated by a UV Transilluminator, but also by a wide range of visible light wavelengths, specifically in the 420-510 nm range.

[Emission Characteristics]

• Upon excitation, RedSafe emits a strong fluorescent signal at 535 nm, which is in the green-yellow region of the visible spectrum.

2. Sensitivity

 

Sensitivity of DNA detection of RedSafe™ Nucleic Acid Staining Solution (20,000x) under UV transmission