TGEV/PEDV RT-PCR Kit

Background Information

Transmissible gastroenteritis (TGE) is a common viral disease of the small intestine that causes vomiting and profuse diarrhea in pigs of all ages. The causal TGE virus infects and destroys villous epithelial cells of the jejunum and ileum, which results in severe villous atrophy, malabsorption, osmotic diarrhea, and dehydration. Porcine epidemic diarrhea (PED) virus diarrhea affects pigs of all ages and clinically resembles TGE in several respects. Oral infection results in viral replication in the epithelial cells of the small intestinal villi. Cells on colonic villi also become infected. No other tissue tropisms have been shown. Virus is excreted in the feces. TGE and PED are highly contagious enteric diseases of piglets. The clinical signs of these diseases are very similar and include watery, yellowish diarrhea.

LiliF™ TGE/PED RT-PCR Kit is able to detect directly and specifically TGE and/or PED by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of TGE and/or PED, allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional RT-PCR method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.