Patho Gene-spin™ DNA/RNA Extraction Kit
Kit Extraccion RNA
A product which extracts DNA/RNA easily and quickly from various pathogenic samples infected by virus or bacteria such as plasma, blood, serum, cell-free body fluids, cell and tissue etc.
• Used low concentration of chaotropic salt and better lysis efficiency
• Higher efficiency of lysis without additional additives
• Stable extraction of DNA/RNA from various pathogenic samples
• Be used safely with non-phenol method and no need for EtOH precipitation
• Suitable for sample extraction such as forensic medicine and diagnosis of diseases etc.
The Patho Gene-spin™ DNA/RNA Extraction Kit uses a low concentration of chaotropic salt and has a better lysis efficiency unlike conventional Total RNA Extraction products. Total DNA/RNA can be extracted from various pathogen samples like virus and bacteria, even anticoagulated plasma/blood, serum, cell-free body fluids and pathogenic cell/tissue etc. And it is easy to induce lysis without any additive like mercaptoethanol. In addition, DNA/RNA extraction is faster and more efficient by using columns that silica-gel membrane technology applied. The DNA/RNA isolated by the Patho Gene-spin™ Kit can be used for clinical diagnosis of people and animals infected with diseases caused by viruses and bacteria.
1. Lysis Buffer is composed high concentration of chaotropic salt. Carefully handle it.
2. Washing Buffer B is supplied as concentrates. Add 40 ml of ethanol (96~100 %) according to the bottle label before use.
Examples of amplification after extractions from various pathogenic samples
After diluting 1/10 of various pathogenic samples, total DNA / RNA was extracted with Patho Gene-spin™ DNA/RNA Extraction Kit. Sensitivity activity and amplification rates were checked using Maxime™ PCR PreMix Kit (i-StarTaq) or Maxime™ RT-PCR PreMix Kit.