PRRSV EU Real-time RT-PCR Kit

Background Information

PRRSV is a small, enveloped RNA virus. It contains a single-stranded, positive-sense, RNA genome with a size of approximately 15 kilobases. The genome contains nine open reading frames. The two genotypes are European type (type I) and North American type (type II). The European and North American PRRSV strains cause similar clinical symptoms, but represent two distinct viral genotypes whose genomes diverge by approximately 40%, thus creating a veil of mystery about the origin of this virus. The genetic variation among the viruses isolated from different places increases the difficulty of developing vaccines against it. Even in the same genotype, high genetic variation among field viruses has been reported all over the world.

LiliF™ PRRSV EU Real-time PCR Kit is able to detect directly and specifically of Porcine Reproductive and Respiratory Syndrome virus(PRRSV EU type) by real-time PCR technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer and probe set for a highly conserved region based on current sequence alignments of EU type of PRRSV, allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the real-time RT-PCR (quantitative) method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.