Taq Polimerasa c/dNTP´s

Taq DNA Polymerase i-Taq – High-Purity Thermostable PCR Enzyme for Reliable DNA Amplification

i-Taq DNA Polymerase is a high-performance thermostable PCR enzyme designed for accurate and reliable DNA amplification in molecular biology laboratories. Additionally, it is engineered to deliver consistent results across a wide range of DNA templates, making it suitable for research, diagnostics, cloning, and routine PCR applications. Therefore, it is widely used in laboratories that require precision, stability, and reproducibility.

Furthermore, this enzyme is purified to remove unwanted bacterial proteins and DNA contaminants. As a result, it ensures higher reaction efficiency and improved amplification quality. In addition, its thermostable nature allows it to withstand repeated heating cycles during PCR without losing activity.

What is i-Taq DNA Polymerase?

i-Taq DNA Polymerase is a 94 kDa thermostable enzyme derived from Thermus aquaticus. Moreover, it is expressed in E. coli and purified to high standards to ensure optimal performance in PCR reactions. Therefore, it is suitable for amplifying genomic DNA, plasmid DNA, and cDNA templates.

In addition, the enzyme is optimized with a specialized buffer system that supports strong activity under different reaction conditions. Consequently, researchers can achieve high-quality DNA amplification even with difficult templates.

Key Features

• 94 kDa thermostable DNA polymerase
• High-purity enzyme for accurate PCR amplification
• Removal of E. coli-derived proteins and DNA contaminants
• Suitable for genomic DNA, plasmid DNA, and cDNA
• Optimized buffer for stable enzyme performance
• Reliable amplification across different templates

Applications

i-Taq DNA Polymerase is widely used in molecular biology and biotechnology. For example, it is suitable for standard PCR, gene amplification, cloning, mutation analysis, and diagnostic testing. Furthermore, it is also used in research fields such as forensic science, agriculture, food testing, and environmental studies.

Because of its reliability, it is commonly used in both academic and industrial laboratories. Additionally, it supports high-throughput workflows where multiple samples must be processed efficiently.

PCR Process Overview

PCR (Polymerase Chain Reaction) is a method used to amplify DNA sequences in vitro. First, DNA is denatured at high temperature to separate strands. Next, primers bind to the target sequence during annealing. Finally, the thermostable DNA polymerase extends the DNA strand during the elongation step.

Moreover, this cycle is repeated 30–40 times. As a result, millions to billions of copies of DNA are produced from a very small starting sample. Therefore, PCR has become an essential tool in modern molecular biology.

Why Choose i-Taq DNA Polymerase?

Because of its high purity and thermostability, i-Taq DNA Polymerase provides reliable and reproducible results. However, unlike low-quality enzymes, it maintains strong activity even under demanding reaction conditions. Consequently, researchers can achieve better accuracy and efficiency in their experiments.

In addition, its optimized formulation reduces non-specific amplification and improves overall PCR performance. Therefore, it is a cost-effective solution for routine laboratory use.

Internal Links

Explore more laboratory products such as
PCR Reagents,
DNA Extraction Kits, and
Molecular Biology Enzymes.

External Resources

Additionally, you can learn more about PCR technology from
NCBI and
Thermo Fisher Scientific.

Product Video