,

TOXO PCR Kit

Availability:

18 disponibles


High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions 94 KDa thermostable DNA polymerase

  • High purity Taq DNA Polymerase
    – Removal of E. coli -derived proteins and DNA that may act as PCR sources
  • Applicable to DNA from cloned DNA to human genomic DNA
  • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • Pack Promo 5×500 units.Código: 25022-5

 

199,00

18 disponibles

Background Information

Toxoplasmosis, a disease of cats and other mammalian species, is caused by a parasitic protozoan, Toxoplasma gondii. Protozoa are single-celled organisms that are among the simplest creatures in the animal kingdom. Although infection with Toxoplasma is fairly common, actual disease caused by the parasite is relatively rare. Most cats show no clinical signs of infection with Toxoplasma. Occasionally, however, clinical disease-toxoplasmosis-occurs, kittens and young adult cats being more often affected than older animals. Lethargy, depression, loss of appetite, and fever are typical early nonspecific signs. Pneumonia, manifested by respiratory distress of gradually increasing severity, is the outstanding sign in many cats. Inflammation of the pancreas and enlargement of lymph nodes also occur. Toxoplasmosis can also affect the eyes and central nervous system, producing inflammation of the retina or anterior ocular chamber, abnormal pupil size and responsiveness to light, blindness, incoordination, heightened sensitivity to touch, personality changes, circling, head pressing, twitching of the ears, difficulty in chewing and swallowing food, seizures, and loss of control over urination and defecation.

LiliF™ TOXO PCR Kit is able to detect directly and specifically Toxopalsma gondii by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Toxopalsma gondii, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.

• This product is optimized for a general purpose PCR machine with a 0.2 ml PCR tube thermal block. Based on the genetic database of the causative Toxopalsma gondii, the reaction product is formed by specific primers of Toxopalsma gondii. After completion of the reaction, confirm whether a band of target size is detected using an electrophoresis and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No.contents48 tests/kit
1TOXO Detection Premix48 tubes
2Positive Control25 μl x 3 tubes
3DNase/RNase Free Water1 ml x 1 tube

Descargar protocolo

 IDR-RP0040_i-Taq.pdf

SKU: IP21666 Categorías: , Etiqueta: