,

FCoV RT-PCR Kit

Availability:

18 disponibles


High-purity i-Taq™ PCR core kit that displays stable and efficient DNA amplification regardless of template type and reaction conditions 94 KDa thermostable DNA polymerase

  • High purity Taq DNA Polymerase
    – Removal of E. coli -derived proteins and DNA that may act as PCR sources
  • Applicable to DNA from cloned DNA to human genomic DNA
  • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • Pack Promo 5×500 units.Código: 25022-5

 

199,00

18 disponibles

Background Information

Feline coronavirus (FCoV) is a positive-stranded RNA virus that is infectious to cats worldwide. This virus is part of the Alphacoronavirus 1 species of the Alphacoronavirus genus belonging to the Coronaviridae family of viruses. Alphacoronavirus 1 also includes the canine coronavirus (CCoV) and the porcine transmissible gastroenteritis coronavirus (TGEV). It has two different forms; the FECV (feline enteric coronavirus) that infects the intestines and the FIPV (feline infectious peritonitis virus) that causes the disease feline infectious peritonitis (FIP). Feline coronavirus is typically shed in feces by healthy cats and transmitted by the fecal-oral route to other cats. In environments with multiple cats, the transmission rate is much higher compared to single-cat environments. The virus is insignificant until mutations cause the virus to be transformed from FECV to FIPV. FIPV causes feline infectious peritonitis, for which there is no known cure; treatment is generally symptomatic and palliative only. Two forms of feline coronavirus are found in nature: enteric (FECV) and FIP (FIPV). There are also two different serotypes found with different antigens that produce unique antibodies. FCoV serotype I is the most frequent. Type I, that can be defined as ‘FECV that could mutate to FIPV type I’, is responsible for 80% of the infections. Typically, serotype I FCoV cultures are difficult to perform, with few resulting studies. FCoV serotype II is less frequent and is described as FECV type II that can mutate to FIPV type II. FCoV type II is a recombinant virus type I with spike genes (S protein) replacement from FCoV by the canine enteric coronavirus (CCOV) spikes. The type II cultures are generally easier to perform, which has resulted in an imbalance of experiments performed with many studies about type II (even though it is a far less common form).

LiliF™ FCoV RT-PCR Kit is able to detect directly and specifically feline coronavirus or feline infectious peritonitis virus by CLP™ technology and Maxime™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of feline coronavirus or feline infectious peritonitis virus, allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional RT-PCR method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative RT-PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes RT-PCR specificity and sensitivity through the control of non-specific priming.

• The assay is a RT-PCR that discriminates feline coronavirus or feline infectious peritonitis virus in one reaction. The assay is composed of two principal steps: (1) nucleic acid extraction from specimens, and (2) amplification of the target extracted nucleic acid fragment using specific primers pair.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No.contents48 tests/kit
1FCoV Detection Premix48 tubes
2FCoV Positive Control25 μl x 3 tubes
3DNase/RNase Free Water1 ml x 1 tube

Descargar protocolo

 IDR-RP0040_i-Taq.pdf

SKU: IP12176 Categorías: , Etiqueta: