FCV RT-PCR Kit

Background Information

Feline calicivirus (FCV), member of the family Caliciviridae, genus Vesivirus is a major cause of acute and chronic upper respiratory diseases in cats. Commonly seen clinical signs include rhinitis, conjunctivitis, stomatitis, gingivitis and glossitis. Less often pneumonia, fever, abortion, cystitis and limping are seen. After resolution of the infection, apparently healthy cats may shed virus for several months or even years. Prevalence rates of feline calicivirus in cats without clinical symptoms vary between 2.5 and 25%. These asymptomatic carrier cats are believed to contribute to the epidemiology of feline calicivirus infection and disease. Recently, virulent feline calicivirus strains associated with a hemorrhagic-like fever and mortality up to 60% in adult cats have been described. Feline calicivirus vaccines have been used widely in the field for the last 20 years. Despite these efforts, feline calicivirusrelated illnesses still present a major problem in the feline population especially for cats living in groups.

LiliF™ FCV RT-PCR Kit is able to detect directly and specifically calicivirus by CLP™ technology and Maxime™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of calicivirus, allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional RT-PCR method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.