FeLV RT-PCR Kit

Background Information

Feline leukemia virus (FeLV) infection is responsible for more deaths among cats than any other infectious disease. The virus affects domestic cats and occurs in some wild felines as well. There are three main types of feline leukemia virus: FeLV-A, FeLV-B, and FeLV-C. FeLV-positive cats can be infected with one, two, or all three types: FeLV-A occurs in all FeLV-infected cats and causes severe immunosuppression (weak-ened immune system). FeLV-B occurs in about 50% of all FeLV-infected cats and causes more neoplastic disease (i.e., tumors and other abnormal tissue growths) than cats infected only with FeLV-A. FeLV-C occurs in about 1% of FeLV-infected cats and causes severe anemia. After the initial infection, the virus replicates in the tonsils and pharyngeal lymph nodes (the pharynx is the muscular tube in the neck). Then it spreads via the bloodstream to other parts of the body, especially the lymph nodes, bone marrow, and intestinal tissue, where it continues to replicate. Viremia, the presence of virus in the blood, usually shows up 2 to 4 weeks after the initial infection.

LiliF™ FeLV RT-PCR Kit is able to detect directly and specifically feline leukemia virus by CLP™ technology and Maxime™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of feline leukemia virus, allowing the RNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional RT-PCR method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.