• Low Background interference
• 10 ~ 200 ng / ml protein quantitative optimization
• Simple application within 10 min
Protein quantification is one of the important steps in protein research like Western blot assay. Protein assay methods are diverse, and there is one which absorbs at 280nm without special reagents or operation. This method is simple. It also has a short coming, not being able to analyze protein assay when amino acid such as phenylalanine, tryptophan or tyrosine are not present. Neither will it be used commonly due to its DNA absorbance interference (AI). The other methods are lowery assay when amino acid such as phenylalanine, tryptophan or tyrosine are not present. Neither will it be used commonly due to its DNA absorbance interference (AI). The other methods are Lowry assay or BCA assay, which are highly sensitive for protein quantification, but is quite inconvenient and time-consuming. Contrarily, Bradford method is the most commonly used one (within 10min), which can detect the minimum amount of protein and is very simple to use. This PRO-MEASURE™ Solution is even more convenient than Bradford assay, declining all of solution itself so that the background absorbance is maintained very low.
Fig 1. Bradford assay principle
Preparation of standard curve using BSA concentration
Using PRO-MEASURE ™ Protein Measurement Solution, dilute by the concentration of BSA provided in the product and measure the absorbance at 595 nm to create a standard curve
Calculate standard curve through OD
595 and concentration of BSA.